20 Questions 56 Answers 0 Followers
Questions related from Héctor Osorio-Vega
I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...
28 June 2024 5,678 1 View
For latex nanoparticles, conjugation to proteins can be done either covalently or non-covalently. The covalent method is usually long (days) and complex (NHS, cabodiimides, etc), but the...
03 October 2023 6,153 2 View
I am trying to find at least 1 good article that describes clearly the molecular mechanism behind protein precipitation with PEG6000. For some reason I'm not able to find thin information clearly...
26 September 2023 5,112 4 View
Hi, I'm wondering what ways you use for improving the clearing of conjugate from nitrocellulose. my device has to use a large amount of gold conjugate and at the end, my NC is left with a...
08 February 2022 9,374 1 View
I know doing this can be complicated but I would really like to know if there is a way to convert the unit copies/ml to ng/ml if we apply it to a specific organism (SARS-cov-2). Any ideas on how...
10 July 2020 3,304 9 View
Hi, I'm making 40nm colloidal gold and following the protocol I always use but now everytime I do it a thin solid film of gold appears on top of the colution and even if I remove it via filtration...
29 May 2020 4,633 3 View
What are the issues preventing us to find a high quality antigen rapid test (immunochromatography) against COVID-19
22 April 2020 1,567 7 View
I'm testing a new protocol for latex beads (150 nm) and I used latex to prevent floculation and enhance mobility. My experiements show that tween 20 prevents conjugation. Anyone has observed this?
06 March 2020 8,564 1 View
Hi. I am looking for dyes (preferably green or blue) that are very stable (time and temperature), do not change color between pH 6-9 and do NOT interact with proteins or sugars. Thanks
23 January 2020 4,792 5 View
I want to know if there has been any study about how permeable can be the capsule that surrounds tumors. what is it made of?
11 August 2016 6,574 17 View
I want to know if there is a method to selectively destroy the mitochondria (DNA and structure) without killing the cell.
23 July 2016 1,068 8 View
What ways are there to find a mutation on a specific gene "in vivo"? Que formas existen para detectar la presencia de una mutacion en un determinado gen sin necesidad de extraer el tejido de un...
30 January 2016 641 4 View
We now know that highly infectious novel viruses can just catch us severely under prepared and that most nations tend to cut funding for healthcare emergencies. Quick diagnosis is going to be...
01 January 1970 9,626 4 View
A non-insignificant portion of the world population could be diagnosed as in a state of crisis when it comes of trusting empirical evidence and the advice of scientific institutions. This can...
01 January 1970 6,946 2 View
A few years ago I moved from my job as a biologist in a research institute to a private research manager. Right now I'm looking for ideas on how to collaborate with other researchers in academic...
01 January 1970 8,026 7 View
It is nothing new to say that the current system for publishing scientific articles has issues, mainly with the paywall that it imposes in front of essential information for the advancement of...
01 January 1970 7,199 2 View
Hi. My name is Hector. I'm a biologist with over 5 years of experience working with lateral flow devices (immunochromatography), conjugation of proteins to latex and gold nanoparticles and other...
01 January 1970 3,774 2 View
I'm sure that many of you will relate to that experience of having an antibody that is just of bad quality. It creates problems because of cross-reactivity or non specific interaction and it's...
01 January 1970 5,302 5 View
Several molecules are known as relevant biomarkers for the detection or followup of cancer, however, is difficult to get from research articles which ones are considered as the most useful in a...
01 January 1970 3,064 3 View
Hi. I want to join a group of scientist that focus on specific problems and work out solutions based on their experience, within a group effort format and mainly working on an remote modality, not...
01 January 1970 7,090 0 View
