I am looking for highly sensitive kits to detect HbsAg in Hepg2.2.15 culture supernatant (cell free media) . Cells well be seeded in 96 well plate
Standard drugs(postive control) for evauation of the invivo hepatoprorective activity (In addition to silymarin )
02 March 2017 8,046 1 View
The protocol is for time dependent study for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression (HepG2.2.15, 96 well plate)? 1- Time course (duration of study) to measure...
31 December 2014 4,950 1 View
I am using Monolisa HBsAg ULTRA (Bio-rad, 96 well plate) for analysis of HBsAg expression (cell line HepG2.2.15), My Qs: 1. Stability of HBsAg (25-37 C) 2. The effect of saving HBsAg at -20...
31 December 2014 3,874 2 View
Can I DMSO as solvent to prepare stock solution of different plant extract for antioxidant screening??
10 November 2014 2,840 12 View
After preparation of the susp. ( B-carltene +lineolic acid + tween40) it is essential to be used freshly? What are the required storage conditions ?
07 August 2014 1,684 4 View
I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are: 1) Can i perform the test in a 96...
10 November 2013 233 4 View
What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?
01 February 2013 9,395 6 View
I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?
31 December 2012 8,051 12 View
I want to test a pant extract for anticancer activity in cell culture
31 December 2011 3,910 13 View
Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...
31 December 2011 8,879 8 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View