Standard drugs(postive control) for evauation of the invivo hepatoprorective activity
(In addition to silymarin )
N acetyl cysteine is for acetaminophen hepato toxicity.
The protocol is for time dependent study for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression (HepG2.2.15, 96 well plate)? 1- Time course (duration of study) to measure...
31 December 2014 4,950 1 View
I am using Monolisa HBsAg ULTRA (Bio-rad, 96 well plate) for analysis of HBsAg expression (cell line HepG2.2.15), My Qs: 1. Stability of HBsAg (25-37 C) 2. The effect of saving HBsAg at -20...
31 December 2014 3,874 2 View
Can I DMSO as solvent to prepare stock solution of different plant extract for antioxidant screening??
10 November 2014 2,840 12 View
After preparation of the susp. ( B-carltene +lineolic acid + tween40) it is essential to be used freshly? What are the required storage conditions ?
07 August 2014 1,684 4 View
I am looking for highly sensitive kits to detect HbsAg in Hepg2.2.15 culture supernatant (cell free media) . Cells well be seeded in 96 well plate
10 November 2013 4,446 0 View
I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are: 1) Can i perform the test in a 96...
10 November 2013 233 4 View
What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?
01 February 2013 9,395 6 View
I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?
31 December 2012 8,051 12 View
I want to test a pant extract for anticancer activity in cell culture
31 December 2011 3,910 13 View
Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...
31 December 2011 8,879 8 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 4,474 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View