Can you answer my questions on a protocol for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression?

31 December 2014 4,860 1 View

What is the stability of HBsAg?

31 December 2014 3,791 2 View

Can I use DMSO as solvent to prepare stock solution of plant extract for antioxidant screening ?

10 November 2014 2,757 12 View

What is the stability of a B-carotene/lineolic acid assay?

07 August 2014 1,598 4 View

Looking for highly sensitive HBsAg ELISA Kits

I am looking for highly sensitive kits to detect HbsAg in Hepg2.2.15 culture supernatant (cell free media) . Cells well be seeded in 96 well plate

10 November 2013 4,367 0 View

HBsAg assay/hepg2.215 cell line?

10 November 2013 152 4 View

Suitable oncertrations of plant extract (80% ethanolic extract) for cytotoxicity (MTT method).

What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?

01 February 2013 9,316 6 View

I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?

I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?

31 December 2012 7,966 12 View

Which is the best method for in screening for anticancer activity of plant extract

I want to test a pant extract for anticancer activity in cell culture

31 December 2011 3,828 13 View

Liquid liquid fractionation for plant extract

Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...

31 December 2011 8,790 8 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View