Refrence drugs(postive control) for evauation of the invivo hepatoprorective activity?

Standard drugs(postive control) for evauation of the invivo hepatoprorective activity (In addition to silymarin )

02 March 2017 7,956 1 View

Can you answer my questions on a protocol for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression?

31 December 2014 4,860 1 View

Can I use DMSO as solvent to prepare stock solution of plant extract for antioxidant screening ?

10 November 2014 2,757 12 View

What is the stability of a B-carotene/lineolic acid assay?

07 August 2014 1,598 4 View

Looking for highly sensitive HBsAg ELISA Kits

I am looking for highly sensitive kits to detect HbsAg in Hepg2.2.15 culture supernatant (cell free media) . Cells well be seeded in 96 well plate

10 November 2013 4,367 0 View

HBsAg assay/hepg2.215 cell line?

10 November 2013 152 4 View

Suitable oncertrations of plant extract (80% ethanolic extract) for cytotoxicity (MTT method).

What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?

01 February 2013 9,316 6 View

I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?

I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?

31 December 2012 7,966 12 View

Which is the best method for in screening for anticancer activity of plant extract

I want to test a pant extract for anticancer activity in cell culture

31 December 2011 3,828 13 View

Liquid liquid fractionation for plant extract

Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...

31 December 2011 8,790 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View