After preparation of the susp. ( B-carltene +lineolic acid + tween40) it is essential to be used freshly? What are the required storage conditions ?
Yes you can use it . also if you want to storge it no problem.. you can put it in deep freeze .
The mixture could be packaged in sealed bags with nitrogen or without oxygen and stored in deep freezer until use
The mixture is very susceptible to oxidation. Therefore, you should use it freshly.
Oils and fats are susceptible to oxidation. Traditionally, chemically synthesized compounds, such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and ter-butyl hydroquinone (TBHQ), are widely used as antioxidants in oil products. β-carotene-linoleic acid assay. In this assay, antioxidant capacity is determined by measuring the inhibition of the volatile organic compounds and the conjugated diene hydroperoxides arising from linoleic acid oxidation Stability is expressed as the oxidation induction time (h), that can be measured with the Rancimat apparatus (Metrohm AG, Herison, Switzerland) using an oil sample of 2 g, warmed to 120° C and a purified air flow rate of 20 l/h.
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