Ahmed H. Arbab

11 Questions 13 Answers 0 Followers

Questions related from Ahmed H. Arbab

Refrence drugs(postive control) for evauation of the invivo hepatoprorective activity?

Standard drugs(postive control) for evauation of the invivo hepatoprorective activity (In addition to silymarin )

03 March 2017 8,020 1 View

What is the stability of HBsAg?

I am using Monolisa HBsAg ULTRA (Bio-rad, 96 well plate) for analysis of HBsAg expression (cell line HepG2.2.15), My Qs: 1. Stability of HBsAg (25-37 C) 2. The effect of saving HBsAg at -20...

01 January 2015 3,847 2 View

Can you answer my questions on a protocol for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression?

The protocol is for time dependent study for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression (HepG2.2.15, 96 well plate)? 1- Time course (duration of study) to measure...

01 January 2015 4,927 1 View

Can I use DMSO as solvent to prepare stock solution of plant extract for antioxidant screening ?

Can I DMSO as solvent to prepare stock solution of different plant extract for antioxidant screening??

11 November 2014 2,816 12 View

What is the stability of a B-carotene/lineolic acid assay?

After preparation of the susp. ( B-carltene +lineolic acid + tween40) it is essential to be used freshly? What are the required storage conditions ?

08 August 2014 1,658 4 View

Looking for highly sensitive HBsAg ELISA Kits

I am looking for highly sensitive kits to detect HbsAg in Hepg2.2.15 culture supernatant (cell free media) . Cells well be seeded in 96 well plate

11 November 2013 4,421 0 View

HBsAg assay/hepg2.215 cell line?

I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are: 1) Can i perform the test in a 96...

11 November 2013 208 4 View

Suitable oncertrations of plant extract (80% ethanolic extract) for cytotoxicity (MTT method).

What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?

02 February 2013 9,373 6 View

I am asking about the sources/causes of contamination in cell culture (in different cancer cell lines ) ,and how to overcome/ treat this problem ?

01 January 2013 8,025 12 View

Liquid liquid fractionation for plant extract

Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...

01 January 2012 8,852 8 View

Which is the best method for in screening for anticancer activity of plant extract

I want to test a pant extract for anticancer activity in cell culture

01 January 2012 3,882 13 View