Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

Myeloperoxidase Colorimetric Activity Assay Kit (Sigma-Aldrich, ELISA MAK068). When do we stop the reaction?

We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.

17 January 2021 6,744 2 View

Quantitative research - hypothesis formulation necessary?

Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...

29 July 2020 6,145 5 View

Does anybody know if there exist research about a possible impact from military used electromagnetic waves frequencies on insects or VLF-waves?

I am very interested in the possible impact of radiation, espacially electromagnetic waves on insects. I searched several times in different online libraries, if there exists any research about...

29 April 2020 5,918 2 View

Could the new starlink satellites be dangereous for the insects due to the electromagnetic waves which they sent to the surface?

There are now many new satellites and are becoming very more ( 30 000) in the next future. I read in the internet that this satellites have not ready investigates risks on healthy and nature due...

23 April 2020 3,660 5 View

Why do I get different protein concentrations by homogenizing?

I implemented a method for protein extraction of skin samples. I did not change the procedure but over time the protein yield decreased. I homogenized skin biopsies using a bead mill homogenizer...

16 April 2020 3,170 7 View

Can anyone recommend a database for dynamic facial expressions that also include expressions of complex emotions?

Most of the databases of dynamic stimuli for facial expressions only include faces expressing basic emotions. Is anyone aware of a database that also includes dynamic face expression stimuli on...

05 November 2019 8,263 2 View

Approved abbreviations for common rat strains?

Hello, I would like to ask if anyone is aware of an existence of an approved abbreviations for common RAT strains. I know that such list is available for the MICE inbred strains, and would like...

08 May 2019 8,103 1 View

Where to publish in the field of cultural management/ academic galleries and museums/ interdisciplinary cooperation/ international networking?

Dear all, I am at the beginning of my academic career and I would like to ask you for your help and experiences? I am interested into peer- reviewed journals with impact (at least Web of...

05 April 2019 9,457 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View