05 April 2017 2 5K Report

Any lymphoma line will do. 

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How many samples do I need, which agar and DNA sequencing technique is most preferable when carrying out microbial diversity in three water bodies?

I am planning to conduct a research project on the microbial diversity in three different water bodies and I would like to know the optimal number of times I should sample each water body to...

06 June 2024 9,946 1 View

What do to change profil?

What o do when I lost ma password and want to change my mail adress as beein retired?

24 April 2024 8,633 1 View

How to optimize Nested PCR ?

I am looking for help to optimize a nested PCR from Long Range (LR) PCR (DNA as template). The LR PCR product looks spesific on agarose gel. We have tried various dilutions of this product as...

01 April 2024 6,835 5 View

Do citizens consider that EU has enough impact on their lives to encourage them to go and vote?

In the last Eurobarometer, 70% of the répondant said that EU had an impact on their lives. But is there a causal effect between saying that the EU impacts our lives, and going to vote? Do...

03 February 2024 2,686 2 View

Will the increasingly frequent volcanic eruptions in the future cool the climate down or warm it up even more?

There are studies by aerosol researchers who have proven that ash particles from volcanic eruptions can temporarily cool down the climate. There are now also reports on the Internet that claim...

10 December 2023 791 3 View

What is the best software for modified BET model to provide phase change diagram, composition and melting temperatures of salt hydrates eutectics?

What is the best software for modified BET model to provide phase change diagram, composition or proportions and melting temperatures of eutectic salt hydrates mixtures phase change materials?

19 November 2023 7,883 0 View

What is the best software or application for simulating eutectic mixture ratios of salt hydrates as PCM?

Looking for best software for simulating binary or termitary salt hydrates ratios as PCM for thermal storage for medium and high temperatures application

22 October 2023 8,342 2 View

Quality assessment regarding learning apps? are there state controlled quality assessments in your country?

How you educators assess quality criteria regarding learning apps? Why are teachers choosing their apps for their teaching, regaring which quality criteria in your country?

14 June 2023 6,973 4 View

What the meaning of rate capability and specific capacity, or is it the same ?

definition how to calculate, especially for lithium titanate

07 June 2023 1,665 2 View

What happens to papers published as part of the 'projects'?

In light of the announcement that the projects feature is going to be removed, I could not find any clear information on what is going to happen to the papers that were uploaded to RG as part of a...

23 February 2023 9,124 5 View

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07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

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How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

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Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

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