Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

Myeloperoxidase Colorimetric Activity Assay Kit (Sigma-Aldrich, ELISA MAK068). When do we stop the reaction?

We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.

17 January 2021 6,744 2 View

Quantitative research - hypothesis formulation necessary?

Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...

29 July 2020 6,145 5 View

Does anybody know if there exist research about a possible impact from military used electromagnetic waves frequencies on insects or VLF-waves?

I am very interested in the possible impact of radiation, espacially electromagnetic waves on insects. I searched several times in different online libraries, if there exists any research about...

29 April 2020 5,918 2 View

Could the new starlink satellites be dangereous for the insects due to the electromagnetic waves which they sent to the surface?

There are now many new satellites and are becoming very more ( 30 000) in the next future. I read in the internet that this satellites have not ready investigates risks on healthy and nature due...

23 April 2020 3,660 5 View

Why do I get different protein concentrations by homogenizing?

I implemented a method for protein extraction of skin samples. I did not change the procedure but over time the protein yield decreased. I homogenized skin biopsies using a bead mill homogenizer...

16 April 2020 3,170 7 View

Can anyone recommend a database for dynamic facial expressions that also include expressions of complex emotions?

Most of the databases of dynamic stimuli for facial expressions only include faces expressing basic emotions. Is anyone aware of a database that also includes dynamic face expression stimuli on...

05 November 2019 8,263 2 View

Approved abbreviations for common rat strains?

Hello, I would like to ask if anyone is aware of an existence of an approved abbreviations for common RAT strains. I know that such list is available for the MICE inbred strains, and would like...

08 May 2019 8,103 1 View

Where to publish in the field of cultural management/ academic galleries and museums/ interdisciplinary cooperation/ international networking?

Dear all, I am at the beginning of my academic career and I would like to ask you for your help and experiences? I am interested into peer- reviewed journals with impact (at least Web of...

05 April 2019 9,457 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View