We have been succesfully using pre-cast Novex NuPAGE Mini Gels (8X8X0.1cm) as a pre-separation and sample visualization step prior to nanoLC/MS/MS for e.g. protein ID. Now we would like to move to a smaller format, ideally shorter and/or thinner. Of course we could shorten the runtime on the gel, but in different hands that will be a somewhat subjective approach. Are there commercially available formats with smaller and/or thinner pre-cast gels that people are successfully using? We are currently using 4-12% gels and Coomassie staining as a generic approach.
Hi Christof,
The smallest gel system I know of are PhastGels. I have used these systems for 3-5yrs but eventually I moved to NuPage (and now BOLT) gels.
PhastGel Gradient – 4-15 http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences/products/AlternativeProductStructure_16708/17067801
You will need completely new equipment however to run them.
What is your aim for doing this? Sounds like you want a faster run and stain time?? Most systems I have tried are around 1-2 hrs to run and visualise... for a reasonable cost. The Phast gels run in 30 mins and visualise in 90mins (at least that was the protocol we were using). NuPage will also run in 30-50mins and visualise in 30-60mins using Coomassie.
Thanks
Russell
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