Can DMSO be a positive control in a necroptosis assay?

Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...

21 June 2018 2,136 2 View

Flow Cytometry protocol for Tregs detection (CD4+, CD25high, FoxP3+) in fresh isolated horses PBMCs ?

Hello, I'm Trying to mark Tregs population in Horses, I adapted a protocol used to mark Tregs in dogs, and It worked at the beginning. But after 7 months I started my experiment and I haven't...

25 May 2018 7,953 5 View

Do you usually submit presubmission enquiries? What are the advantages and what do you usually write to get the best feedback?

Do you think it is something worth doing?

28 April 2018 7,654 7 View

Western Blot bands change after second antibody exposure.

Dear all, I have an interesting problem. I performed a WB but the initial primary antibody incubation produced weak bands after 20 minute exposure. So I reincubated with the same antibody solution...

14 May 2017 9,484 2 View

Can anyone suggest research papers on Social Science gamification?

I need some papers about Gamification but in Social Science

06 July 2016 3,144 0 View

How do I reduce the self-fluorescence of cells of Ehrlich ascites carcinoma while performing an assay of DCFH-DA, in flow cytometry?

13 April 2013 8,401 6 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View