Can DMSO be a positive control in a necroptosis assay?

Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...

21 June 2018 2,136 2 View

Flow Cytometry protocol for Tregs detection (CD4+, CD25high, FoxP3+) in fresh isolated horses PBMCs ?

Hello, I'm Trying to mark Tregs population in Horses, I adapted a protocol used to mark Tregs in dogs, and It worked at the beginning. But after 7 months I started my experiment and I haven't...

25 May 2018 7,953 5 View

Looking for a Toloudine blue protocol for skeletochonology analyses?

Hi, I am looking for a Toloudine blue protocol for skeletochronology analyses. I will use lizard femurs, bones will be embedded in paraffin. Specimens were injected with 10% formalin, and are...

04 May 2018 6,746 0 View

Do you usually submit presubmission enquiries? What are the advantages and what do you usually write to get the best feedback?

Do you think it is something worth doing?

28 April 2018 7,654 7 View

Western Blot bands change after second antibody exposure.

Dear all, I have an interesting problem. I performed a WB but the initial primary antibody incubation produced weak bands after 20 minute exposure. So I reincubated with the same antibody solution...

14 May 2017 9,484 2 View

How do I reduce the self-fluorescence of cells of Ehrlich ascites carcinoma while performing an assay of DCFH-DA, in flow cytometry?

13 April 2013 8,401 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View