How to quantify the benzene by HPLC method?

I want to know how to quantify the benzene by using HPLC method (any isocratic method)? I performed with methanol and water (80: 20) as mobile phase, but i am not getting any incremental height...

10 February 2020 7,888 4 View

Can DMSO be a positive control in a necroptosis assay?

Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...

21 June 2018 2,136 2 View

Flow Cytometry protocol for Tregs detection (CD4+, CD25high, FoxP3+) in fresh isolated horses PBMCs ?

Hello, I'm Trying to mark Tregs population in Horses, I adapted a protocol used to mark Tregs in dogs, and It worked at the beginning. But after 7 months I started my experiment and I haven't...

25 May 2018 7,953 5 View

Looking for a Toloudine blue protocol for skeletochonology analyses?

Hi, I am looking for a Toloudine blue protocol for skeletochronology analyses. I will use lizard femurs, bones will be embedded in paraffin. Specimens were injected with 10% formalin, and are...

04 May 2018 6,746 0 View

Do you usually submit presubmission enquiries? What are the advantages and what do you usually write to get the best feedback?

Do you think it is something worth doing?

28 April 2018 7,654 7 View

Western Blot bands change after second antibody exposure.

Dear all, I have an interesting problem. I performed a WB but the initial primary antibody incubation produced weak bands after 20 minute exposure. So I reincubated with the same antibody solution...

14 May 2017 9,484 2 View

Can anyone suggest research papers on Social Science gamification?

I need some papers about Gamification but in Social Science

06 July 2016 3,144 0 View

What is the purpose of a conference publication?

What is the purpose of a conference publication (with peer-reviewed full paper, indexed in popular repositories such as Google scholar, Scopus, dblp, etc.)? I am wondering about the purpose of the...

01 January 1970 8,669 6 View

Systematic review and meta-analysis

I am doing a systematic review and meta-analysis where my subject of interest are the staff nurses. In one study which i am including in the review, the subjects are the doctors and the nurses....

01 January 1970 7,924 8 View

How to draw a forest plot for systematic review and meta analysis?

hi. i am doing a systematic review and meta-analysis. the studies included are two-group pre-post quasi-experimental. all the studies have ONLY reported the pre-post test mean and SD. is it...

01 January 1970 738 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View