Looking for a Special Issue on "Oxidative Stress and Mitochondrial Function as Targets to Modulate Cancer Cell Behavior and Chemoresistance"?

Oxidative Stress and Reprogramming of Mitochondrial Function and Dynamics as Targets to Modulate Cancer Cell Behavior and Chemoresistance Open Special Issue on Hindawi's Oxidative Medicine and...

09 October 2018 7,239 0 View

Has anyone used the SIRT1 Activity fluorometric Assay Kit from Abcam?

Hi everyone. I am considering buying the SIRT1 Activity fluorometric Assay Kit from Abcam (cat. no. ab156065). However, after reading very carefully the protocol booklet, I am quite dubious. What...

04 May 2018 1,662 4 View

Has anyone used DRP1 (Phospho-Ser616) antibody from Biorbyt?

Hi. I am currently studying how certain treatments can worsen cancer cell behaviour through the alteration of mitochondrial dynamics. Literature reports show that mitochondrial fission is...

04 May 2018 7,016 2 View

Open Special Issue on OMCL - Mitochondria as a Central Hub in the Cellular Response to Redox-Active Environmental Pollutants ?

Open Special Issue on Oxidative Medicine and Cellular Longevity Mitochondria control critical cellular functions, including redox homeostasis, apoptosis, and autophagy. Several pathologies arise...

31 December 2017 3,372 2 View

Uneffective treatments with resveratrol or SRT1720 for SIRT1activation. What's happening in my HUVECs?

Hi everyone. I 've just tested two classic SIRT1 activators (resveratrol and SRT1720) in my HUVECs. Both compounds were dissolved in DMSO, and the final concentration ranges were 0-20 microM (RV)...

11 December 2017 5,796 6 View

Non-specific antibody binding or detection of complexed/immature forms of GSTA2?

Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...

09 October 2017 6,777 7 View

Does anyone know the rate of disappearance of H2O2 in an acqueous solution?

06 July 2016 4,464 7 View

Band choice in SIRT1-directed Western blotting?

04 May 2016 5,936 12 View

Possible non-parametric alternative to a three-way ANOVA. Any suggestion?

03 April 2016 3,859 19 View

Weird phenomenon: spontaneous Western blot revelation and brown band appearance. How could that be possible?

02 March 2016 7,620 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View