14 Questions 38 Answers 0 Followers
Questions related from Stefano Falone
Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...
06 June 2019 2,827 3 View
Oxidative Stress and Reprogramming of Mitochondrial Function and Dynamics as Targets to Modulate Cancer Cell Behavior and Chemoresistance Open Special Issue on Hindawi's Oxidative Medicine and...
10 October 2018 7,363 0 View
Hi everyone. I am considering buying the SIRT1 Activity fluorometric Assay Kit from Abcam (cat. no. ab156065). However, after reading very carefully the protocol booklet, I am quite dubious. What...
05 May 2018 1,769 4 View
Hi. I am currently studying how certain treatments can worsen cancer cell behaviour through the alteration of mitochondrial dynamics. Literature reports show that mitochondrial fission is...
05 May 2018 7,124 2 View
Open Special Issue on Oxidative Medicine and Cellular Longevity Mitochondria control critical cellular functions, including redox homeostasis, apoptosis, and autophagy. Several pathologies arise...
01 January 2018 3,488 2 View
Hi everyone. I 've just tested two classic SIRT1 activators (resveratrol and SRT1720) in my HUVECs. Both compounds were dissolved in DMSO, and the final concentration ranges were 0-20 microM (RV)...
12 December 2017 5,893 6 View
Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...
10 October 2017 6,872 7 View
Hi. I know that hydrogen peroxide undergoes spontaneous decomposition when dissolved in an acqueous solution. According to some literature I found, H2O2 would have a half-life of ~5-10 hrs,...
07 July 2016 4,563 7 View
Hello.This is the membrane I got after WB and chemiluminescence detection (68 seconds) on my human neuroblastoma SH-SY5Y cells extracted in RIPA+anti-phosphatase/protease (anti-SIRT1,...
05 May 2016 6,047 12 View
Hi,I have just carried out an experiment in which I measured a dependent variable in cells that were divided in 8 groups. +Magnetic field, +NAC, +H2O2 +Magnetic field, -NAC, +H2O2 +Magnetic...
04 April 2016 3,986 19 View
Hi everyone, a very weird phenomeon is occurring in my lab. In order to detect some proteins that are expressed at low level I am using an ECL kit (for high level-expressed proteins I normally use...
03 March 2016 7,747 13 View
Hi everyone, I asked the supplier of my experimental animals about the age when CD-1 female mice may be considered "reproductively old". I was told that "reproductors" undergo replacement when...
11 November 2015 1,996 3 View
Hi,I bought the STA-811 kit (OxiSelect™ Methylglyoxal (MG) Competitive ELISA Kit) to measure the methylglyoxal-dependent protein damage in my human plasma/serum samples, though I do not know...
06 June 2015 8,898 1 View
I get no amplification but I see a very high fluorescence (normal ROX profile) already before the first PCR cycle (raw data: 1x10^6 fluo units). Such a high fluorescence declines...
09 September 2014 936 5 View
