What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

05 June 2019 2,726 3 View

Looking for a Special Issue on "Oxidative Stress and Mitochondrial Function as Targets to Modulate Cancer Cell Behavior and Chemoresistance"?

Oxidative Stress and Reprogramming of Mitochondrial Function and Dynamics as Targets to Modulate Cancer Cell Behavior and Chemoresistance Open Special Issue on Hindawi's Oxidative Medicine and...

09 October 2018 7,239 0 View

Has anyone used the SIRT1 Activity fluorometric Assay Kit from Abcam?

Hi everyone. I am considering buying the SIRT1 Activity fluorometric Assay Kit from Abcam (cat. no. ab156065). However, after reading very carefully the protocol booklet, I am quite dubious. What...

04 May 2018 1,662 4 View

Open Special Issue on OMCL - Mitochondria as a Central Hub in the Cellular Response to Redox-Active Environmental Pollutants ?

Open Special Issue on Oxidative Medicine and Cellular Longevity Mitochondria control critical cellular functions, including redox homeostasis, apoptosis, and autophagy. Several pathologies arise...

31 December 2017 3,372 2 View

Uneffective treatments with resveratrol or SRT1720 for SIRT1activation. What's happening in my HUVECs?

Hi everyone. I 've just tested two classic SIRT1 activators (resveratrol and SRT1720) in my HUVECs. Both compounds were dissolved in DMSO, and the final concentration ranges were 0-20 microM (RV)...

11 December 2017 5,796 6 View

Non-specific antibody binding or detection of complexed/immature forms of GSTA2?

Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...

09 October 2017 6,777 7 View

Does anyone know the rate of disappearance of H2O2 in an acqueous solution?

06 July 2016 4,464 7 View

Band choice in SIRT1-directed Western blotting?

04 May 2016 5,936 12 View

Possible non-parametric alternative to a three-way ANOVA. Any suggestion?

03 April 2016 3,859 19 View

Weird phenomenon: spontaneous Western blot revelation and brown band appearance. How could that be possible?

02 March 2016 7,620 13 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View