Why I see no changes in hardness of AlCu alloy after 2 months natural aging?

08 December 2020 2,929 4 View

How to detect ethyl acetate and acetaldehyde in ethanol solution?

Hello everyone, does any one know any method to detect the existence of ethyl acetate and acetaldehyde in an ethanol medium? I have currently taken UV-VIS is there any method to detect the...

02 November 2019 1,665 3 View

Request to introduce of good resources?

25 September 2019 322 2 View

How sulfide concentration is measured by standard spectrophotometric method?

Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...

18 November 2017 8,325 3 View

Can we use Thioflavin T (ThT) staining for recognizing aggregated beta amyloid in lysed cell (caco2 cell line)?how can I do it?

I want to detect kinetic of beta amyloid aggregation in present of lysed cell of caco2 cell line and in present of beta amyloid? please help me

09 May 2016 9,842 3 View

Are there any structural benefits of constructing double shell domes?

I am working on Iranian Domes. There are many double-shell domes in Iran. I know that making double-shell dome helps heat insulation, but I wonder if there is any structural benefit of making...

01 February 2016 9,589 7 View

Does anyone have experience with Chimera software for RMSD calculation?

I am going to calculate RMSD between native (from PDB) and my docked complexes. I opened complexes in ArgusLab, removed their proteins, deleted all hydrogen (H) to have analogues with the same...

20 June 2015 8,875 9 View

Why when I use commercial HRP and TMB as substrate, not giving expected blue color?

Hi dear researchers We used HRP (Biobasic) in phosphate buffer (pH: 6.5) and after adding TMB, the reaction turning to light blue without adding H2O2. After adding H2O2 to reaction media, the...

01 January 1970 748 3 View

What is the difference between enzyme immobilized activity yield and recovery?

I am studying enzyme immobilized nanoparticles for catalytic applications. Research are using different names like activity yield or activity recovery. What is the difference between enzyme...

01 January 1970 2,646 12 View

How can I determine pH stability of my immobilized enzyme?

Hi researchers I determine optimal pH and temperature of immobilized enzyme activity. I want to know how pre-incubated it at different pH for pH stability factor.

01 January 1970 6,461 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View