Hello,
I am looking to obtain a contig of atleast 1500 bp to identify fungi on the species level. Is there any publications or recomendations of primers to amplify the ITS region and the flanking regions to obtain a longer read than 400-600 bp?
Dear Christian,
This recent publication might be useful:
http://onlinelibrary.wiley.com/wol1/doi/10.1111/nph.14776/abstract
Article PacBio metabarcoding of Fungi and other eukaryotes: Errors, ...
Best,
vincent
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