Im looking to use H2O2 with my HEK293 cells to cause a stress response in order to see if the cells become more sensitive to externally applied electromagnet fields. I need the cells to still be adherent to my 96 well plate after treatment with H2O2 while still being stressed. Has anyone used H2O2 with HEK cells and still had the cells adhere and if so at what concentration of H2O2?
Hi. I've had them done when studying the protective effect of H. itama honey towards oxidatively stressed HEK293 cells.
The concentration of H2O2 I used to maintain HEK293 cell attachment to the flask is 100 μL of 1 mM. However, I recommend determining the IC50 of the H2O2 you plan to use in your lab, as it may differ from my conditions.
Typically, when inducing oxidative stress, cell death occurs, and as a normal observation, dead cells detach and clump together.
However, if you want to ensure cell attachment during stress, consider the following tips:
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