Alif Ismail

9 Questions 37 Answers 0 Followers

Questions related from Alif Ismail

WST-8 vs MTT?

Hi everyone, Has anyone experienced ambiguous or conflicting results between WST-8 and MTT assays in cytotoxicity studies? I am currently testing two peptide candidates (SKP85 and SKP179) for...

31 May 2025 8,587 3 View

Peptide solvent for cell-based Assay?

Hello. I am trying to test my designed peptide binding towards MDA-MB-231 and MCF-7 cell lines. However, I first need to dissolved my peptides as they are in lyophilized form. The suggestion from...

28 October 2024 8,989 1 View

How to start with peptide design?

Hello, I am wondering if any of you experts could help me with my peptide design. I want to design a peptide that can penetrate the skin cell and specifically target the epidermal growth factor...

02 April 2024 3,218 1 View

How to install AlphaFold2?

Hi everyone. I am actually very new to protein computational so I have zero idea how do I install AlphaFold 2 to be run on my computer. I heard that if you install AlphaFold + all required...

08 March 2024 8,517 3 View

How to normalize data obtained for MTT assay result?

Does anyone knows how to normalize the data obtained for MTT assay result to 100%? Because when I count the %viability I get more than 100% and upon consulting my supervisor, she told me to...

19 June 2023 4,801 6 View

Does this HEK293 contaminated?

Hello everyone. I need help. I am new to cell culture (2 months +) so I am not really experienced to distinguish these cells if they're healthy or not. Is this HEK293 cells contaminated?...

15 June 2023 6,569 6 View

Any reference method for oven-drying method for determination of moisture content of honey sample?

Hello. I am currently looking to study honey sample moisture content. However, due to limitation of devices and equipment, the only feasible way for me to do so is using oven-drying method. Ive...

10 April 2023 4,516 2 View

Is my FC reagent suitable for TPC assay?

Hello everyone. I want to perform TPC assay for phenolic content estimation of my honey samples. However, the only FC reagent available in my lab is from Sigma Aldrich that got 'suitable for...

22 March 2023 314 3 View

Relative Antioxidant Capacity Index?

Hello everyone. I am currently working on comparing antioxidant potency between my samples. I have 4 samples = water kefir, water kefir infused with butterfly pea, water kefir infused with...

21 January 2023 5,167 5 View