I've been running lots of gels in my lab and the ladder is usually stored in the -20 Freezer, but I left it out at RT on the bench overnight. Would this have any effect on its resolution/etc for future use?
I would expect it to be absolutely fine. DNA is very stable at room temperature and overnight is probably not long enough for bacterial action to be a problem. On some old markers made by restriction digestion sometimes the bands re annealed a little and the ladder looked fuzzy and in this case I suggest heating the ladder to 60c briefly which often improved resolution but modern markers are are not made this way so I expect it to run well
Hi there,
100pb and 1kb DNA ladders are always on the bench in my lab to avoid repeated thaw/freeze cycles. Never been in trouble with that.
It should be just fine. My graduate lab would just leave it on the bench until it was used up.
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