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Questions related from Alex Dimitri
So I deleted an exon of a gene in a cell line using CRISPR. The region deleted is around 1500bp. I isolated colonies, extracted DNA and ran a PCR with primers designed upstream and downstream of...
06 December 2018 3,539 2 View
I've been running lots of gels in my lab and the ladder is usually stored in the -20 Freezer, but I left it out at RT on the bench overnight. Would this have any effect on its resolution/etc for...
02 October 2017 7,376 3 View
I'm currently setting up immunostaining of prepared slides. The protocol usually is: Let primary antibody incubate at 4C overnight, then the next day add the secondary body on for 2 hours at RT. I...
22 June 2017 9,665 11 View
I was wondering if there is a reason to use one method over the other, depending on what you're looking and staining for or depending on the cell type? From what I know, methanol denatures and...
01 June 2017 4,897 8 View
