Anyone knows which I should use as a reducing agent for anaerobic microbiology media: L-cysteine HCL Anhydrous or Monohydrate? Or it won't make a difference?
Every single recipe I found only specify L-Cysteine HCL, but nothing more.
Hi Gunes-Helene, we use the monohydrate for reducing media and indicate only this compound in our recipes. The main reason is the price. The monohydrate is about ten-times cheaper than the water-free compound.
Thank you all very much for your answers! It helped a lot in choosing the right reducing agent. And I will keep in mind the effect of the HCL salt on the ph of my media and adjust accordingly.