Gunes-Helene Isitan

11 Questions 17 Answers 0 Followers

Questions related from Gunes-Helene Isitan

Suggestion for a software for in-silico PCR that gives band pattern?

Hi! Anyone knows of a software that can generate band patterns from whole genomes and custom primers? I have seen it for digestion patterns, but could not find one for PCR. My primers have some...

13 July 2021 7,220 2 View

How to keep pre-poured anaerobic plates?

Hi! I have a very simple "novice-with-anaerobic-culture" question. I want to pre-pour my anaerobic plates to be used over two weeks. I usually keep aerobic pre-poured plates in the walk-in fridge,...

03 June 2019 8,948 1 View

Any suggestion of clear soil/gel soil recipe for seed germination AND root observation?

Hi! I am looking for a recipe for a type of clear soil to see roots formation, but that would also resist/slightly resist fungal/bacterial contamination. It is a project for kids at a science...

30 March 2019 7,193 12 View

Using expired plastic ware in the lab (tips, micro tubes, etc.)?

Hi! Does anyone have experience with expired plastic ware in the lab, especially pipette tips??? I know sterility might become an issue, but one can always autoclave. But I also know plastic...

11 September 2018 2,752 6 View

Spin columns: difference between those for plasmids, pcr product, virus and genomic DNA??

Hi! I've been looking for replacement spin columns for purifying PCR product from 16s bacterial DNA. I have found several "different" ones online: some for plasmid DNA, some for genomic DNA, some...

20 July 2018 1,215 4 View

Mammalian mtDNA isolation: sucrose gradient or spin columns?

Hi! I've been trying to isolate complete mtDNA plasmids from cheek cells (16.5kbp). Once the nuclear DNA is pelleted and the supernatant containing the mtDNA is transfered in a new tube, pelleted,...

26 May 2018 332 3 View

DNA extraction protocol for anaerobic bacteria? (Gram + and -)?

Hi! I have been trying to extract DNA from anaerobic bacteria (both gram positive and negative) using the lyzozyme/proteinase K/sds/chloroform/isopropanol protocol, but have repeatly failed to get...

27 April 2018 7,419 4 View

Pharmacia EPS 3500 XL electrophoresis power supply leads?

Hi! Ok, that may be a very basic question to ask this community, but I have searched and searched and searched some more, and can't seem to find the answer! What are the required adapters to...

22 April 2018 4,449 0 View

L-Cysteine HCL Anhydrous or Monohydrate?

Hi! Anyone knows which I should use as a reducing agent for anaerobic microbiology media: L-cysteine HCL Anhydrous or Monohydrate? Or it won't make a difference? Every single recipe I found only...

09 April 2018 7,482 3 View

Possible pathogens present in healthy people's gut microbiota?

Hi! I have been asked (Health & Safety department) to provide a list of possible pathogens that could be grown from healthy human stools as a risk assessment for an upcoming research-creation...

18 January 2018 7,475 2 View

Paramecium and electricity: why do they stop responding to galvanotaxis after a while?

Hi, I am working on an interactive art installation that offers contact zones between human and non-human. In this specific case, my non-human are Paramecium Caudatum and Multimicronucleatum. I am...

28 March 2016 9,394 5 View