You can use classical quantification techniques which includes Colony forming unit (CFU) counting and crystal violet staining. Many other modern methods like ATP bioluminescence, Quartz crystal microbalance, Characterization of biofilm morphology with use of scanning electron microscopy and spectroscopic methods, use of software like ImageJ etc for biofilm analysis have been used by many researchers.

You can refer to the following articles:

1. Wilson, C., Lukowicz, R., Merchant, S., Valquier-Flynn, H., Caballero, J., Sandoval, J., ... & Clement, B. (2017). Quantitative and qualitative assessment methods for biofilm growth: A mini-review. Research & reviews. Journal of engineering and technology, 6(4).

2. Kırmusaoğlu, S. (2019). The Methods for Detection of Biofilm and Screening Antibiofilm Activity of Agents. In Exopolysaccharides-Methods of Preparation and Application. IntechOpen.

You can use classical quantification techniques which includes Colony forming unit (CFU) counting and crystal violet staining. Many other modern methods like ATP bioluminescence, Quartz crystal microbalance, Characterization of biofilm morphology with use of scanning electron microscopy and spectroscopic methods, use of software like ImageJ etc for biofilm analysis have been used by many researchers.

You can refer to the following articles:

1. Wilson, C., Lukowicz, R., Merchant, S., Valquier-Flynn, H., Caballero, J., Sandoval, J., ... & Clement, B. (2017). Quantitative and qualitative assessment methods for biofilm growth: A mini-review. Research & reviews. Journal of engineering and technology, 6(4).

2. Kırmusaoğlu, S. (2019). The Methods for Detection of Biofilm and Screening Antibiofilm Activity of Agents. In Exopolysaccharides-Methods of Preparation and Application. IntechOpen.

You can find your answer in my paper:

Inhibitory effect of zinc oxide nanoparticles on pseudomonas aeruginosa biofilm formation

Crystal violet staining of biofilm mass is the direct method to quantify the biofilm mass and you can also calculate the biofilm inhibition (indirectly) by counting planktonic cells by CFU determination.

Best

Abhishek Dharm Singh Could you help me downing the two reference you refered on December 26, 2019 ? I could download them,please send them to my E-mail: [email protected],Thank you!