The instrument is old, I changed a lot of the other plumbing. The pressure is fine and steady when equilibrating, then when it goes to make the injection, it just starts flowing from the needle and not through the brand new needle seat...
Thanks, it ended up being the rheodyne valve, it was sticking so I had to take it apart, clean it and replace the stator.
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
12 June 2024 7,331 1 View
Dear community, I can't seem to find any clear information on power analyses work so I'm throwing my question out onto the internet hoping for answers. :) Study Details I am doing a...
19 May 2024 9,214 4 View
Here's the brief: 1. I synthesized alkyl gallates (ethyl gallate, butyl gallate, and amyl gallate) using Fischer esterification. 2. I used TsOH instead of concentrated H2SO4 as the catalyst. 3....
03 May 2024 6,737 5 View
Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...
23 April 2024 6,637 0 View
Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...
31 March 2024 5,920 4 View
Although there have been a number of initiatives, the shortage of drinking water is still a problem for some communities. In this context, we can see that people's need for water is increasing as...
26 February 2024 5,285 3 View
Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...
25 February 2024 2,181 3 View
Hello everyone, I receive many emails about different journals and publishers inviting manuscripts (some predatory, some not); however, received something new. Someone from Science Paths sent an...
12 December 2023 1,819 0 View
According to a report by the Sustainable Development Solutions Network (SDSN) published in 2023, only 18% of SDG targets will be met by 2030. Should we expect a new global agenda?
26 November 2023 7,921 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View