Jonathan Decenzi

3 Questions 28 Answers 0 Followers

Questions related from Jonathan Decenzi

Does anyone have advice on how to remove starch from samples I am running for sugar using HPLC/RID?

I am running several matrices for sugar on the HPLC, and no matter how clear the sample looks, the pressure jumps when it gets to this particularly starchy sample. I suspect that the starch is...

20 March 2014 9,083 33 View

How important is peak shape to you in method development?

At times, I have run into analytes that don't give particularly great peak shape, whether on GC, HPLC or MS. I have run methods where despite some tailing on the chromatography, we have...

26 October 2013 910 3 View

Just changed the needle and seat on an Agilent 1100, the pressure went down when equilibrating, but now drops to nothing on injection

The instrument is old, I changed a lot of the other plumbing. The pressure is fine and steady when equilibrating, then when it goes to make the injection, it just starts flowing from the needle...

24 April 2013 7,740 1 View