9 Questions 14 Answers 0 Followers
Questions related from Vincent Blancheteau
Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent
12 December 2016 1,454 3 View
Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince
10 October 2016 5,988 0 View
Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...
10 October 2016 5,881 1 View
Hello, Can anyone share microscopy images showing monocytes/macrophages in human PBMC? or point me towards articles/resources showing them? many thanks.
09 September 2016 8,663 3 View
Hello, Quick question: in vitro, could I get a T cell activation/proliferation if I stimulate human PBMC with anti-CD3+IL-2? thanks vince
09 September 2016 5,313 11 View
At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...
07 July 2016 9,288 3 View
How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?
07 July 2016 6,340 7 View
Hello, What are the relative proportions of the sub-populations in mouse spleen, LNs, Blood? any article or link to recommend? Many thanks.
10 October 2015 7,093 2 View
Hello, I would like to setup a proliferative assay, I don't have access to irradiation. So instead of using irradiated splenocytes for an thymidine incorporation assay, would CFSE/CD4 staining be...
09 September 2015 5,985 0 View
