[flow cytometry] txt or cvs file to FCS file?

Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent

11 December 2016 1,418 3 View

What is fluorochrome photostability/photodegradation?

Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...

09 October 2016 5,845 1 View

Jurkat T cells TCR specificity?

Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince

09 October 2016 5,870 0 View

Mono/macro in human PBMC?

Hello, Can anyone share microscopy images showing monocytes/macrophages in human PBMC? or point me towards articles/resources showing them? many thanks.

08 September 2016 8,630 3 View

How to get T cell proliferation within human PBMC?

08 September 2016 5,258 11 View

How can I improve the sensitivity and dynamic range for my competitive ELISA?

At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...

06 July 2016 9,256 3 View

How long does an antibody take to interact with its target antigen ?

How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?

06 July 2016 6,285 7 View

Relative proportions of sub-populations in mouse spleen, LNs, blood?

Hello, What are the relative proportions of the sub-populations in mouse spleen, LNs, Blood? any article or link to recommend? Many thanks.

09 October 2015 7,059 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View