[flow cytometry] txt or cvs file to FCS file?

Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent

11 December 2016 1,418 3 View

What is fluorochrome photostability/photodegradation?

Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...

09 October 2016 5,845 1 View

Jurkat T cells TCR specificity?

Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince

09 October 2016 5,870 0 View

How to get T cell proliferation within human PBMC?

08 September 2016 5,258 11 View

How can I improve the sensitivity and dynamic range for my competitive ELISA?

At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...

06 July 2016 9,256 3 View

How long does an antibody take to interact with its target antigen ?

How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?

06 July 2016 6,285 7 View

Relative proportions of sub-populations in mouse spleen, LNs, blood?

Hello, What are the relative proportions of the sub-populations in mouse spleen, LNs, Blood? any article or link to recommend? Many thanks.

09 October 2015 7,059 2 View

Would CFSE/CD4 staining be fine in an assay where all splenocytes are restimulated?

Hello, I would like to setup a proliferative assay, I don't have access to irradiation. So instead of using irradiated splenocytes for an thymidine incorporation assay, would CFSE/CD4 staining be...

08 September 2015 5,952 0 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View