Hello,
What are the relative proportions of the sub-populations in mouse spleen, LNs, Blood?
any article or link to recommend?
Many thanks.
Dear Vincent,
Check if these papers give you the information you need. You can consult the authors on RG:
Article Normal Structure, Function, and Histology of the Spleen
Article Presence of antibody-forming cell precursors in the mouse ad...
Article The separation of sub-populations of T and B lymphocytes
Article Chronodependent Effect of Interleukin-2 on Mouse Spleen Cell...
many thanks
Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent
11 December 2016 1,489 3 View
Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...
09 October 2016 5,918 1 View
Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince
09 October 2016 6,083 0 View
Hello, Can anyone share microscopy images showing monocytes/macrophages in human PBMC? or point me towards articles/resources showing them? many thanks.
08 September 2016 8,706 3 View
Hello, Quick question: in vitro, could I get a T cell activation/proliferation if I stimulate human PBMC with anti-CD3+IL-2? thanks vince
08 September 2016 5,359 11 View
At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...
06 July 2016 9,318 3 View
How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?
06 July 2016 6,381 7 View
Hello, I would like to setup a proliferative assay, I don't have access to irradiation. So instead of using irradiated splenocytes for an thymidine incorporation assay, would CFSE/CD4 staining be...
08 September 2015 6,015 0 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...
01 August 2024 6,469 6 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View