[flow cytometry] txt or cvs file to FCS file?

Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent

11 December 2016 1,418 3 View

What is fluorochrome photostability/photodegradation?

Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...

09 October 2016 5,845 1 View

Jurkat T cells TCR specificity?

Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince

09 October 2016 5,870 0 View

Mono/macro in human PBMC?

Hello, Can anyone share microscopy images showing monocytes/macrophages in human PBMC? or point me towards articles/resources showing them? many thanks.

08 September 2016 8,630 3 View

How to get T cell proliferation within human PBMC?

08 September 2016 5,258 11 View

How can I improve the sensitivity and dynamic range for my competitive ELISA?

At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...

06 July 2016 9,256 3 View

How long does an antibody take to interact with its target antigen ?

How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?

06 July 2016 6,285 7 View

Would CFSE/CD4 staining be fine in an assay where all splenocytes are restimulated?

Hello, I would like to setup a proliferative assay, I don't have access to irradiation. So instead of using irradiated splenocytes for an thymidine incorporation assay, would CFSE/CD4 staining be...

08 September 2015 5,952 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View