07 October 2016 1 612 Report

I'm using 100% methylation & 0% methylation plasmid for standart control. Then, we mix them to get 75%, 50%, 25% & 10% standart. Then, we convert with BIsulfite modification and run in qPCR 7500 Fast AB machine. But, unfortunately, the melting curve didn't show good gradient that we hope.

Any suggestion?

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