I'm running MS-HRM PCR for Nasopharyngeal Cancer marker, I have an issue with melting curve of control standart ?

I'm using 100% methylation & 0% methylation plasmid for standart control. Then, we mix them to get 75%, 50%, 25% & 10% standart. Then, we convert with BIsulfite modification and run in...

07 October 2016 629 1 View