Mammary glands in Platypus ?

Dear researchers, Can anyone help me to identify these odd mammary glands in Ornithorynchus anaticus ? I struggle to understand its mecanism : The secretion from all these flat lobes have a...

03 March 2020 8,275 2 View

Does anybody know any kind of preservation media for endometrial biopsy suitable for flow cytometry analysis?

I'm interested in any kind of preservation media for endometrial biopsy which could be stored at room temperature for at least up to 3 or 4 days. I've tested RNAlater media, but it seems to fix...

06 November 2018 1,845 2 View

Which is the best analytic column to separate antibodies and its variants?

I am looking for an analitic column to evalute the hidrophobicity profile of different antibodies and its variants. High resolution and high selectivity it is need, since am I interested in really...

10 September 2018 1,705 6 View

What's this peak?

Hi, i've injected 5 microliters of pure CHCl3 in a 250 mm C18 column, with ACN:H2O 80:20 as solvent, 1,0 ml/min. Something appears at 3,72 min, absorbing strongly at 210 nm. Is the CHCl3 absorbing...

20 December 2017 8,768 17 View

What do you think about this peak tailing?

21 March 2017 7,981 13 View

How to get dN/dS values for each branch?

I'm trying to test the selection model in an alignment of 40 coding nucleotide sequences, with its respective ML tree. I would need to get the dN/dS values for each branch of the tree but I'm...

24 December 2013 7,458 2 View

Does anybody know an automated method to quantify microglia?

Does anybody know an automated method to quantify microglia or has experience with the one described in the attached article? I would like to use it and would appreciate some...

25 September 2012 4,561 9 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View