We purchased an Elisa Kit for Human Insulin and the manufacturer indicated the use of Heparin/Lithium tubes. Unfortunately, the samples were collected in Heparin/Sodium tubes. Will the results vary too much?
We have contacted the manufacturer, but their response left us unsure.
Dear Viviana,
It is generally recommended to follow the manufacturer's instructions and use the specific type of tubes or collection vessels specified in the Elisa kit protocol. Different types of tubes or collection vessels may have different properties that could affect the accuracy and reliability of the Elisa assay.
For example, the type of anticoagulant present in the tubes or collection vessels may influence the stability and activity of the analyte (in this case, human insulin) being measured. Heparin and lithium are commonly used anticoagulants in Elisa assays, and they may affect the stability of the analyte differently. If you have collected your samples in Heparin/Sodium tubes, it is possible that this could affect the accuracy of the Elisa assay.
It is always a good idea to carefully follow the manufacturer's instructions and use the recommended tubes or collection vessels to ensure the accuracy and reliability of the Elisa assay. If you are unsure about the suitability of your samples or the potential impact on the assay results, it is best to consult with the manufacturer or a technical support representative for guidance. They may be able to provide additional information or recommendations to help you obtain the most accurate and reliable results.
Yours sincerely,
Edgar M Cambaza
Edgar Manuel Cambaza
What is he purpose of posting texts generated by GPT?
https://openai.com/blog/chatgpt/
I would not expect any major problems, esp. when you compare different samples for relative amounts, but absolute quantitation could be an issue. Check how the standards are prepared, if they do contain any serum and tube components. To be on the safe side, directly compare samples drawn with both kind of tubes and/or spike some samples with known amounts of insulin.
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