Hi Bobbi! There are several ways you could try, although do bear in mind that adult astrocytes will be less plastic and more difficult to culture than neonatal. A good method for adult would be immuno-panning for astrocytes, either positively (I.e) with anti-astrocytes antibodies or negatively, using anti microglia and oligodendrocyte antibodies (it's unlikely neurones would survive from adult tissue!). You could immuno-pan either by immunobilising the antibody on dishes or beads. In fact, Miltenyi sell bead-based kits for doing just this! You could check out Foo et al, 2011 (Ben Barres Lab) for a nice paper on this subject. Hope that helps Bobbi! Very best wishes, Marc.

Thanks Marc. That is indeed the protocol that we are going to trial. I wondered whether I had missed any alternatives, but hopefully this is good enough. Thanks for the reference ! :-)