Reverse engineering a protein - kind of?

I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide. So, I know that the molecular weight of my...

29 October 2018 3,907 3 View

SV40 immortalised human microglia from ABMgood ?

17 February 2016 8,364 2 View

Does anyone know where I can get the list of genes which are known downstream targets for the transcription factor CEBPB?

Using Enricher I can see that my gene set contains 235/3478 genes known to be targets of CEBPB via the Transfac/Jasper tab. Does anyone know where i could get the list of all the CEBPB genes so...

10 February 2016 1,814 3 View

Does anyone know of research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses?

17 November 2015 9,908 3 View

Is it beneficial to use ELISA to quantify p53 levels in cultured cell fractions?

ELISA and western blots are only as good as the antibodies used to make them - but despite a plethora of published westerns for measuring p53 there are very few reports using ELISA. I have...

21 November 2012 8,474 14 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

No title

02 March 2021 3,060 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View