Hello, I am wondering if anyone has any journal papers that outline extraction, purification and characterization techniques that could be done by an advanced undergraduate laboratory class in 3-4 lab periods? I have a couple; extraction of myoglobin from ground beef, extraction of phycocyanin from spirulina, HRP from horseradish peroxidase and lysozyme from egg white. Are there any others? I would like to be able to offer 6-10 papers for students to choose from for independent projects in which they must replicate the work of the paper, but only have 3 (others are incorporated into previous classes.
Thank you in advance!
Dear Bobbi S Stromer
I have a few to add to your list.
1. Extraction, Purification, and Characterization of Lycopene from tomato.
Article Extraction, Purification, and Characterization of Lycopene f...
2. Extraction of Chlorophylls from Spinach Leaves.
Article Cloud Point Extraction of Chlorophylls from Spinach Leaves U...
3. Extraction and purification of carotenoids from vegetables.
https://www.jocpr.com/articles/extraction-and-purification-of-carotenoids-from-vegetables.pdf
4. Isolation of casein from milk.
https://egyankosh.ac.in/bitstream/123456789/71253/1/Experiment-5.pdf
Hope these will be helpful!
Best.
I developed these for a Biochemical Engineering class, looking at alkaline phosphatase production and purification during phosphate-limited growth.
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