7 Questions 22 Answers 0 Followers
Questions related from Marc Watson
A few papers in the last 3-4 years have described protocols for deriving microglia, or at least microglia-like cells from mouse ES cells (Beutner et al, 2010; Napoli et al, 2009). These techniques...
19 December 2014 7,908 2 View
Do optogenetic tools and constructs, like the classic channelrhodopsins, have usage restrictions that stop non-academic research labs from freely utilising them for research purposes? Thanks everyone.
24 September 2014 5,711 5 View
Does anybody have any advice on the quality and functionality of the two high content imagers and their associated analysis software above? Image quality, ease of analysis, reliability etc. Many...
25 March 2014 7,692 4 View
I've been culturing primary E18 rat cortical and hippocampal neurons for years but does anybody have any good tips to cleanly remove ganglionic eminence/striatum out of the same tissue for primary...
18 January 2014 5,570 1 View
I have a question about the A2B5 antibody and its use for staining oligodendrocyte precursor cells, that I'd be really interested to hear your experiences and expertise about. I’m aware that A2B5...
29 October 2013 7,717 4 View
I've been culturing mixed glia and getting microglia from them for years, but it's always struck me that they look slightly yellow under the microscope. If you take a lysate of microglial cell as...
21 August 2013 5,465 9 View
Does anybody have any views on the best methods for differentiating rat oligodendrocytes in vitro please? My feeling is that as soon as they start to mature passed the OPC stage a large subset...
21 July 2013 7,925 11 View
