Bobbi Fleiss

11 Questions 62 Answers 0 Followers

Questions related from Bobbi Fleiss

Reverse engineering a protein - kind of?

I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide. So, I know that the molecular weight of my...

30 October 2018 4,174 3 View

Human brain tissue from adults born preterm ?

Does anyone know of a brain bank or researcher that will have brain tissue from patients that were born preterm? Is it something that is recorded?? thanks!

09 May 2016 9,177 5 View

SV40 immortalised human microglia from ABMgood ?

Has anyone used them? (https://www.abmgood.com/productSearch.php?searchQuery=T0251) I have been in contact with the authors on the cited paper (http://www.ncbi.nlm.nih.gov/pubmed/25561230) but I...

18 February 2016 8,532 2 View

Does anyone know where I can get the list of genes which are known downstream targets for the transcription factor CEBPB?

Using Enricher I can see that my gene set contains 235/3478 genes known to be targets of CEBPB via the Transfac/Jasper tab. Does anyone know where i could get the list of all the CEBPB genes so...

11 February 2016 1,947 3 View

Does anyone know of research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses?

Does anyone know of any research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses (or similar)? Thanks!

18 November 2015 10,112 3 View

Could you please update me as to the best way to try and get immortalised human microglia?

Hi everyone, Most recent contact to get HMO6 or SV40 microglia. I have heard that contacting the authors for HM06 can be difficult. Also, does anyone know how much the SV40 is, the website to buy...

27 August 2014 9,612 7 View

Anti-Iba1 antibody (ab5076) : Does anyone have any experience with it in mouse or monkey?

We are looking for a good alternative to the Rabbit-Anti-Iba1 from Wako as although it it great it limits the use of other rabbit antibodies for double labelling. Does anyone have any experience...

17 September 2013 9,684 12 View

Isolating astrocytes from adult brain without FACS

Can anyone tell me if there are protocols for the separation of astrocytes that can be used in adult animals that do not require a FACS. Thanks !!

22 August 2013 3,040 2 View

Species differences - qRT-PCR ?

We are running qRT-PCR in mouse, pig and monkey. We find that we have very low CT values for multiple genes that we expect will be very high - reference genes (GAPDH, YWAHZ) - and inflammatory...

02 July 2013 3,229 0 View

Best gel agent for holding (post-mortem) small animal tissues in place during MRI

I am imaging mouse brains in a small bore 9.4 and need a gel (agarose ?) to set the brain in the correct position with a 50ml tube. Can anyone please recommend the best one to use.

27 December 2012 9,818 3 View

Is it beneficial to use ELISA to quantify p53 levels in cultured cell fractions?

ELISA and western blots are only as good as the antibodies used to make them - but despite a plethora of published westerns for measuring p53 there are very few reports using ELISA. I have...

22 November 2012 8,662 14 View