Isolate and preserve sciatic nerve in order to check autoantibodies binding by immunofluorescence ?

More Chloé Bost's questions See All

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Suitable psychology/sociology journals for long papers?

Could someone please recommend a social psychology or gender studies journal that accepts long papers or doesn't have an upper word count limit?

22 June 2024 4,125 3 View

How do I change my email?

I am retiring, but still interested in academic work. How can I change the email to which you send notifications? Thanks.

12 May 2024 6,436 1 View

What implications does elevated LDH activity from underfilling have on the clinical analysis of laboratory findings and patient diagnoses?

I would like to know the reason why preventing the production of underfilled tubes for LDH testing is important.

06 February 2024 6,746 2 View

How to handle J774A ?

I've just defrosted some J774A for the first time. On thawing, the cells are small, round and not at all adherent... Is this normal? Do I need to add collagen to make them adhere to the support...

18 January 2024 211 1 View

How to structure a web-based content analysis of Fashion Brands?

The aim of my research is to elucidate the what moral discourse is evident in the communication of the fashion brand Golden Goose regarding the intentional distressing of fashion items (the...

17 January 2024 8,179 1 View

Working with macrocharcoal in the tropic region, do you recommend using Charanalysis?

I understand charanalysis has been applied to mostly USA forests, threshhold values work the same in the tropic region? Has anyone tested this program for the tropical region.

12 December 2023 1,346 0 View

What is the usual concentration of Methyl Orange dye in textile wastewater?

Need this for my research please also indicate which article it was from, thank you.

20 November 2023 1,500 2 View

What is the best way to store Grubbs 2 catalyst long term?

I’ve been storing Grubbs 2 catalyst (Sigma Aldrich, M204) inside the glovebox at room temperature for the past 6 months but only been using it extensively for 3 months. I’ve realized recently that...

09 November 2023 6,955 0 View

How to count THP-1 cells ?

I'm working on THP-1 monocytes. I thawed them last week to start my culture, but I can't count them. I count in Mallassez cells with trypan blue. I can see by microscopy that my cell density is...

26 October 2023 1,598 3 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View