Is unconjugated bilirubin soluble in0.02M NaOH,without albumin?

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Henrik Rasmus Andersen

How could you have pH 7.4 and 0.02 M NaOH (pH ~12.3)?

Lucie Muchova

Yes, it is soluble. The solubility of unconjugated bilirubin in aqueous environment is pH dependent, at pH 12.3 it is very well soluble due to ionization of COOH groups but it is unstable. See also a very good review by Don Ostrow (Journal of Lipid Research (1994)35:1715)

Matteo Dal Ben

Yes. We usually dissolve bilirubin in 0.01 N NaOH.

We dissolve for immediate use only and not for storage in NaOH.

It's also soluble in DMSO.

Betty Titus Mathew

Thank you all for the answer.

i need to check the stability of bilirubin bounded to albumin. in PBS solution.How can i do that .when taking spectrophotometric readings from 0 hour to six or 24 hours it shows variations