Name of Assay: Albumin Denaturation Assay
There is no trend in the data, so there must be some technical problems with the assay. One possibility is that the inhibitor is interfering with the measurement due to effects such as insolubility or optical interference.
Dear Prof, is there a subtle distinction between the terms "substitution" and "doping" when referring to modifying ceramic dielectric materials for example Na0.5Bi0.5TiO3? Is there any difference...
08 July 2024 2,043 3 View
Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...
07 July 2024 3,526 3 View
we need surface area, corrosion current, equivalent weight and density of the metal involved for corrosion rate calculation in mm/year (lets say). For dissimilar metal welds, how can we measure...
30 June 2024 5,943 2 View
Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...
29 June 2024 10,023 1 View
Pharmacokinetic and pharmacodynamic differences, adjustments drug dosages in children to ensure safe and effective therapy.
21 June 2024 7,491 1 View
I want to know about the journals that don't charge for the publication of an article and also the one that takes less time to process.
20 June 2024 9,211 0 View
I do a research about Non-Verbal Communication: Gestures and Body Language in Classroom.
18 June 2024 8,737 1 View
In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...
17 June 2024 3,888 2 View
Im currently writing my Thesis on Challenges/ Problems/ Particularities regarding Dark Tourism and have a little trouble finding the right literature. I have found a lot of literature for dark...
14 June 2024 7,262 2 View
Action research design is Quantitative Studies or Qualitative Studies?
14 June 2024 1,684 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View