Albumin salting out by ammonium sulphate protocol ?

09 February 2024 1 5K Report

Hello,

I seek the protocol for the albumin salting out by ammonium sulfate.

if I understand it correctly, you want to precipitate albumins by ammonium sulphate addition. All proteins precipitates in the saturated ammonium sulphate solution which is 4.1 M at room temperature.

Badges
Science topic

More Asma Foufou's questions See All

What are gaps in diabetic wound healing techniques or dressings formation research?

Wound Healing research gaps using some polymers

28 July 2024 5,178 0 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Je veux des articles et des ouvrages en rapport avec mon sujet de recherche?

voilà les mots clés: la communication ,sage femme; technolecte

27 May 2024 1,740 1 View

Is there anyone can help me with finding some samples of source codes or designed projects for OMnet++, SUMO, VANET Routing Protocol ?

I am trying to compare the performance of routing protocol like AODV, GPSR , DSDV , DYMO in VANET using OMNET++

19 May 2024 8,866 2 View

About subtypes of Dyslexia : Single article containing: "Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...?

I am unable to find a source containing all subtypes of dyslexia ("Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...) in a single article to use as a reference for my...

27 March 2024 8,454 2 View

How to extract data from figures with high accuracy?

Such as studies that represent results (mean, standard deviation) with graphs rather than numerical data

08 March 2024 3,555 1 View

Apoptosis assay using flow cytometry, can I use annexin FITC in GFP fluorescent cells?

I have transfected cells with GFP, which emit in close range to FITC and used apoptosis kit that have annexin conjugated with FITC to measure apoptosis and PI to measure death. I am gating for GFP...

07 March 2024 9,673 5 View

How to recover single strand dna after AMpure XP Beads purification ?

Hello! I have a problem with my purification step. I have a single strand dna (after a digestion using Lambda Exonuclease) that I need to purify before injecting it as a dna donnor in C.elegans...

06 March 2024 5,529 0 View

Do you suggest that I order my kit from BT Lab?

I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.

27 February 2024 6,940 1 View

If i mix magnetite and maghemite nanoparticle using silica as matrix does it consider as nanocomposite?

to find a research gap i want to mix mnagnetite and maghemite nanoparticle and mix them with silica as matrix does it considered as nanocomposite?

23 February 2024 2,205 1 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,917 7 View

Asked a separate ways?

Dear all, I have a question regarding ways to separate ammonium nitrate and phosphorus pentoxide？

12 July 2024 3,164 2 View

Does the potentiostat have to be in a fume hood when polymerising the PANI?

I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...

10 July 2024 2,936 3 View

65% saturation of ammonium sulfate in 100 ml? (for protein precipitation)?

Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...

09 July 2024 5,508 0 View

I want to ask why the poly aniline film does not stick to the ITO type substrate knowing that I used the electrodeposition method?

In order to make a polyaniline film, I used the electrodeposition method and set all the conditions, but I noticed that the polymerization process is happening, but it doesn't stick to the...

09 July 2024 2,708 2 View

How to catch or detect Herbivore‐induced plant volatiles from a plant?

I want to know about herbivore-induced plant volatiles from a plant attacked by pests. Is there any chance for me to know what kinds of compounds the plant produces and emits in real time? Thank...

27 June 2024 5,161 5 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

Ow can I remove the fat from mustard seeds in order to extract the bioactive compounds from the de-oiling residue?

I need to eliminate the fat fraction in order to obtain a better product in the next stage of bioactive extraction. By processing the mustard seeds as they are, I obtain a sub-optimal result in...

05 June 2024 3,484 3 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

How exactly do we prepare mobile phases used for LC/MS?

I am doing research that requires using LC/MS to analyse my data. I have very little knowledge of chemistry and LCMS. I have read through many posts and articles, but there is not a single place...

03 June 2024 3,963 5 View