Hello,
I seek the protocol for the albumin salting out by ammonium sulfate.
if I understand it correctly, you want to precipitate albumins by ammonium sulphate addition. All proteins precipitates in the saturated ammonium sulphate solution which is 4.1 M at room temperature.
I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...
18 January 2021 7,240 7 View
In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...
09 January 2021 2,802 5 View
In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...
11 December 2020 4,663 3 View
I would be grateful if you could suggest a paper or book which provides an equation to determine the dimensions (length) of an electrical nanodipole and magnetic loop antenna at desired optical...
17 October 2020 2,421 4 View
How trauma and fear of infection can affect brain functions and structures?
10 October 2020 6,642 1 View
when I blast the existed primers specially 16s rDNAs , I cant get good results.
21 September 2020 1,584 4 View
Hello, I am looking for protcoles to evaluate the following components: TDF : total dietary fiber, SDF : soluble dietary fiber, IDF : insoluble dietary fiber et TDN : total digestive nutrients.
06 September 2020 371 2 View
I am looking for a reputable journal for publication of research article of MS thesis. Kindly suggest me some journals related to water treatment through adsorption which is free of cost.
13 July 2020 8,259 6 View
I want to know if the water included in resin is enough as a solvent or that water is not counted and I should add water as solvent to paint?
21 June 2020 322 6 View
Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)
26 April 2020 3,003 36 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...
23 February 2021 5,645 1 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
I am interested in using TLC to detect non-linear xylooligosaccharides. What would be the best visualisation solvent? Would aniline phthalate solution be suitable? I have also seen people...
02 February 2021 7,748 3 View
Dear researchers, Anticancer peptides(ACPs) with cell-penetrating properties can enter to cytoplasmic space of cancer cells and induce cell death. but, I could not find the database that...
29 January 2021 707 3 View
My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.
22 January 2021 4,693 7 View
Hi, I have been analysing gas phase organic solvents with the help of FTIR. The spectrum looks a bit weird around 1200 cm^-1 (see attached image). I noticed a similar behaviour when the gas cell...
12 January 2021 3,286 8 View
it has been observed that publication of an outcome of a natural herbomineral product in a clinical study contains mechanism of action in terms of chemistry of individual constituents of the...
04 January 2021 7,372 3 View
During the process of aqueous extraction of phenolic compounds, I got a mucilage that must be separated from the extract that I would like to study. Thank you for your help.
03 January 2021 1,765 3 View