I'm isolating RNA from epididymis by using TRIzol, but I'm getting only single band (tried 30+ time and only two time got two bands).

whenever I got single band there was always no amplification. Any one tell me that why I'm troubling with this single band problem while I.m taking all precautions such as DEPE-tips/tubes/ethanol/water and RNAse away for hand & bench area cleanup.

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