Subhash Ph.D.

8 Questions 9 Answers 0 Followers

Questions related from Subhash Ph.D.

How to make sample for ELISA measurement of cytosolic pro-IL3 beta?

Help will be appreciated.

26 October 2023 6,837 0 View

What is the pH of buffalo oviductal fluid (NOT cattle)?

If you have any research article which directly saying pH of oviductal fluid in buffalo, Please share it with me. I really appreciate any help you can provide.

15 May 2023 3,688 5 View

How to replace an amino-acid with glycosylated or phosphorylated amino-acid residue at desired point in protein 3D structure?

Hi, I have found two SNP (Alanine to serine & threonine) at different positions in my desired gene (Sanger sequencing), and then I have built a protein 3D structure by using I-Tasser and...

07 July 2020 4,010 0 View

How to replace bio-informatically an amino-acid with glycosylated/phosphorylated amino-acid in protein 3D (tertiary) structure?

Hi, I have found two SNPs (Alanine to serine & threonine) at different positions in my desired gene (Sanger sequencing), and then I have built a protein 3D (tertiary) structure by using I-Tasser...

07 July 2020 9,400 0 View

How to defense small sample size data and their sub-sampling?

I have taken two groups high fertile and low fertile having 5 bulls in each group. Semen samples were collected from each bull and genomic DNA isolation was done for all animals of both groups and...

11 November 2019 6,086 0 View

Can anyone suggest me mammalian expression vector with Hig-tag and GFP tag?

I want to express a mammalian protein (179 amino acids) in CHO cell line. please suggest me any vector with HIS tag and GFP tag (selection purposes) which will suitable for CHO cells. thank you...

17 September 2019 7,151 4 View

Is tissue's regions responsible for RNA quality? one, two or three bands on agarose gel?

I'm isolating RNA from epididymis by using TRIzol, but I'm getting only single band (tried 30+ time and only two time got two bands). whenever I got single band there was always no amplification....

09 August 2019 9,940 3 View

How to precipitate genomic DNA from a solution of sodium-acetate (pH 4.2)+ethanol(100%)??

I want to precipitate genomic DNA extracted by PCI (Phenol:Chloroform:isoamylalcohol, 25:24:1 ratio) method and for that I mistakenly add sodium-acetate pH 4.2 (instead of pH 5.2) + 100% ethanol,...

26 June 2019 2,390 7 View