That circle one is yeast contamination in cell culture? (file was attached)
Cell proliferation is slow and morphology was changed.
Please tell me how get rid of yeast contamination.
What kind of antibiotics? Gentamycin? Penicilin?
Thank you :)
Yeast are 3 - 5 micron (RBC are ~ 6 - 8 micron) and show characteristic budding (cell division). The agent in your culture looks much smaller than this as estimated from the size of your cultured cells which I suspect are ~ 20 micron +/-. If this is an accurate presumption on my part you culture is contaminated with a bacteria. Perform a gram stain. I suspect it is a gram positive coccus.
As Frank stated, it seems to me a bacterial contamination, possibly of a diplococcus. You may consult your lab colleagues about appropriate use of antibiotics. There may be endemic strains that are resistant to commonly-used antibiotics such as Penicillin-Streptomycin. If you do suspect yeast (fungi) contamination, you should add anti-fungal agents. Anti-bacterial agents do not reliably work on fungi.
If you are worried about fungi you may like to add ~ 250 mg/L fluconazole in combination with your Pen/Strep or whatever you decide to use.
Hi,
Antifungi often induces DNA damages in cells. Then, if morphology and cell growing are already changed, I suggest you to discard the flask, discard you medium bottle and PBS bottle, wash you hood and defrost a new vial of cell :/
This does not look like Yeast.. May be bacteria but the media should turn turbid in that case.. If you are looking for avoiding yeast.. Amphotericin B may work.. For bacteria Pen strep is good enough if you work clean.. Gentamycin could also be used for multiple contaminants.. its broader.. But I would shy away from using too many antibiotics.. Would be better if you use fresh cells..
i highly suggest you to discard these cells and start with a fresh batch, preferably stock that was frozen well before the growth rate began slowing down.
Even if you remove the infection- it may have affected the metabolism of your cells. I use Normocin for extreme cases, otherwise Amphotericin B and strep-pen for routine culturing
Gentamycin is one of the last weapon against bacteria resistant to penicilline/streptomycine. Use it too often is to get the chance to bacteria becoming resistant. After reaching the point (triple strength), contamination of cultures becomes dangerous for the user. Also I highly inadvisable to use such a product.
On this question, I want to add a comment to other friends. Due to the fact that yeasts, like bacteria, quickly yellowish the environment and are slightly larger than them, and it is difficult to detect them from each other, and that you must do the fungus treatment or bacterial treatment so:
Put some of your contaminated environment out of the room. Next day, if the environment is yellow, it is a bacterial contamination, and if the environment is pink, it is contaminated with yeast and the more you put it in the environment, the more pink.
Good luck
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