this is a photo for a callus of date palm (phoenix dactylifera), in this treatment the cal developed a forms like roots, but these forms go upwards not down.
Apparemment c'est la stipe, mais il faudra vérifier par observation microscopique.
It is not surprising to see roots growing upwards, during plant regeneration from monocot tissue cultures. You can verify if these formations are roots if you keep the callus cultures under lights. Normally, root tissues do not show differentiation from proplasts to chloroplasts. So, they should remain white coloured even after some days or weeks.
Mohammed, they are Roots. transfer your culture to half strength medium with low sucrose. with this conditions the roots will grow normally. i also observed the same when i worked on in-vitro studies of Sorghum bicolor
Dear Mohammed,
Very often, we see roots exhibiting ageotrophic or negatively-geotrophic behaviour in tissue cultures, especially in semi-solid medium (irrespective of they are dicots or monocots). If you use liquid medium (using filter paper bridges or fine mesh of luffa or any other cucurbit fruit as support), you may overcome this problem. As a physiologist, I rather would like to stress that root (in general) grow towards water or high moisture. In semi-solid medium the amount of free water available would depend on percentage of agar or any other gelling agent one uses (further, if concentration of gelling agent is high, it may restrict penetration of root into the medium) and generally we find lot of moisture settling on the surface of vessel (due to condensation). Excess of moisture and/or 100% humidity above medium (in vessel) could promote growth of root in ageotrophic or negatively geotrophic manner.
Although, I agree that what you are seeing in your date palm cultures are roots, but they might be accompanied by node or something like node from where primary leaf emerges (you may see few good Youtube videos that depict germination of date palm seeds).
thank you all for your response. I think that is a root in the image, because I waited three weeks and no change in the color of tissue.
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