Column size reduction over height (multistory) RC building ?

dear engineers i have inquiry regarding the reduction of columns over height of reinforced concrete frames is it for strength of architecture or economic purposes ?? and what is the preferred...

24 January 2020 6,094 4 View

What is the protocol for extraction of all theses components (DNA, RNA, Protein, phospholipid, peptidoglycan) from microorganism?

Hello everyone, I hope you a good day I want to ask about a protocol for extraction of the previously mentioned component from the microorganism. Is there an easy an low-cost method to be used I...

03 March 2018 9,983 2 View

GNU Octave + EEGLab in Windows 10?

Dear Colleagues, I was wondering has any of you tried running EEGLab (latest version 14.1.1b) in GNU Octave GUI (latest version 4.2.1) in Windows 10? If so, were you able to run EEGLab GUI? There...

09 October 2017 9,146 0 View

How can I calculate the anneling temp. of the primer?

I used NCBI for designing the primer which I need, but I want to know how can I calculate the annealing temp. of it ? this is the primer that I want to know its annealing temp.? Forward primer...

11 February 2017 9,589 9 View

What is the reason that cause RNA dose not appear on the gel?

09 November 2016 3,708 5 View

What is the protocol of using DEPC in RNA extraction ?

I want to extract RNA by Direct-Zol RNA Miniprep kit from sigma but the kit didn't provide RNase free tubes and one of my colleagues advised me to use diluted DEPC to sterilize normal Eppendorf...

05 October 2016 3,751 6 View

Why blank value is higher than sample value in ELISA test? and Is there any way to avoid negative values after substract sample value from blank?

blank 0.208 values of samples ranges from (0.165-2.2080)a lot of sample values below blank value

26 May 2016 3,555 15 View

Can any one help me for the determination of specific alkaloids in vinca plants as a co-operation between us?

i have a dry plant materials leaves, stem and root? if possible tell me to send the plant materials and we will published the paper together. the paper interested with inducing alkaloid production...

09 May 2016 3,771 2 View

What is a suitable method of dissolving salicylic acid for large a volume to use it in the field?

Thanks in advance for your replies.

26 December 2014 7,852 3 View

What is the IC50 for artemisinin in different stages of P. falciparum gametocytes.

Artemisinins activity against mature gametocytes.

16 September 2013 2,383 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View