Column size reduction over height (multistory) RC building ?

dear engineers i have inquiry regarding the reduction of columns over height of reinforced concrete frames is it for strength of architecture or economic purposes ?? and what is the preferred...

24 January 2020 6,094 4 View

What is the protocol for extraction of all theses components (DNA, RNA, Protein, phospholipid, peptidoglycan) from microorganism?

Hello everyone, I hope you a good day I want to ask about a protocol for extraction of the previously mentioned component from the microorganism. Is there an easy an low-cost method to be used I...

03 March 2018 9,983 2 View

How can I calculate the anneling temp. of the primer?

I used NCBI for designing the primer which I need, but I want to know how can I calculate the annealing temp. of it ? this is the primer that I want to know its annealing temp.? Forward primer...

11 February 2017 9,589 9 View

What is the reason that cause RNA dose not appear on the gel?

09 November 2016 3,708 5 View

What is the protocol of using DEPC in RNA extraction ?

I want to extract RNA by Direct-Zol RNA Miniprep kit from sigma but the kit didn't provide RNase free tubes and one of my colleagues advised me to use diluted DEPC to sterilize normal Eppendorf...

05 October 2016 3,751 6 View

Why blank value is higher than sample value in ELISA test? and Is there any way to avoid negative values after substract sample value from blank?

blank 0.208 values of samples ranges from (0.165-2.2080)a lot of sample values below blank value

26 May 2016 3,555 15 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View