WA Gadalla

7 Questions 6 Answers 0 Followers

Questions related from WA Gadalla

Is there any chance for my c-DNA product as it stay at 85 C for 29 mins?

I have performed a reverse transcription using the High Capacity cDNA RT Kit but due to an error in programming the thermocycler instead of one cycle (25C-10min, 37C-120min, 85C-5min) the last...

19 October 2020 1,689 5 View

Is this pcr product is good enough to do digestion by restriction enzyme?

This is the result of the pcr experiments of some samples, I want to know if it good enough to digest it by RE to detect polymorphism or I should perform PCR again. Also I want to know if...

22 April 2019 202 0 View

What is the protocol for extraction of all theses components (DNA, RNA, Protein, phospholipid, peptidoglycan) from microorganism?

Hello everyone, I hope you a good day I want to ask about a protocol for extraction of the previously mentioned component from the microorganism. Is there an easy an low-cost method to be used I...

04 March 2018 10,065 2 View

How can I calculate the anneling temp. of the primer?

I used NCBI for designing the primer which I need, but I want to know how can I calculate the annealing temp. of it ? this is the primer that I want to know its annealing temp.? Forward primer...

12 February 2017 9,698 9 View

What is the reason that cause RNA dose not appear on the gel?

I am trying to extract RNA from whole blood , first I am collecting the blood on EDTA tube put it in ice box until I reach to my lab this take about 30-45 mins then put it in -20c until second day...

10 November 2016 3,772 5 View

What is the protocol of using DEPC in RNA extraction ?

I want to extract RNA by Direct-Zol RNA Miniprep kit from sigma but the kit didn't provide RNase free tubes and one of my colleagues advised me to use diluted DEPC to sterilize normal Eppendorf...

06 October 2016 3,855 6 View

Why blank value is higher than sample value in ELISA test? and Is there any way to avoid negative values after substract sample value from blank?

blank 0.208 values of samples ranges from (0.165-2.2080)a lot of sample values below blank value

27 May 2016 3,783 15 View