I am maintaining an N2a cell line and I can see black dots that for the most part seem non-moving (a few seem to exhibit Brownian motion) but I am not sure if it's bacterial contamination, cellular debris or possibly something from the serum. The pictures are after I have washed the cells. Thank you for the help.
Did you just defreeze them? if so, it is normal, and after 1-2 passages they should decrease but if after 1-2 passages they stay and increase it could be bacterial or mycoplasma infection
Ra Alb Even if defreezing process was harsh a few of them must have survived from DMSO and grew, After two weeks you definitely washed most of debris, I don't know the exact morphology of your cells but they don't seem ok, if you have several flasks leave of one them in an incubator and don't do anything for 4-5 days. if it's a bacterial infection flask will be full of them, but if it's mycoplasma cells will be so damaged. you can even check your media and serum for any infection, or it can be because of the temperature of your media in passaging procedure was it 37?
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